Reactivity of sorbose dehydrogenase from <i>Sinorhizobium</i> sp. 97507 for 1,5-anhydro-d-glucitol

Purified recombinant sorbose dehydrogenase from <i>Sinorhizobium</i> sp. 97507 exhibited high reactivity for 1,5-anhydro-d-glucitol (1,5-AG) and l-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (<i>k</i>_cat<i>/K</i>_m) for l-sorbose and 1,5-AG is 1.8 × 10² and 1.5 × 10² s⁻¹·M⁻¹, respectively.从中华根瘤菌（Sinorhizobium）菌株97507中纯化得到的重组山梨糖脱氢酶，对1,5-脱水-d-葡萄糖醇（1,5-anhydro-d-glucitol, 1,5-AG）与L-山梨糖表现出高催化活性，但对其余受试糖类及糖醇类物质仅具有极微弱的催化活性。动力学分析结果显示，该酶针对L-山梨糖和1,5-脱水-d-葡萄糖醇的催化效率（k_cat/K_m）分别为1.8×10² s⁻¹·M⁻¹和1.5×10² s⁻¹·M⁻¹。