Characterizing the interactions between a naturally-primed IgA (mAb 260.8) and its conserved Bacteroides thetaiotaomicron species-specific epitope in gnotobiotic mice [array]. Bacteroides thetaiotaomicron

Comparisons of gnotobiotic Rag1-/- mice, with and without subcutaneous 260.8 hybridomas, disclosed that this IgA does not affect B. thetaiotaomicron population density or suppress 260.8 epitope production but does affect bacterial gene expression in ways that are emblematic of a diminished host innate immune response. Overall design: C57BL/6 wildtype, C57BL/6J Rag1-/- , and C57BL/6J Rag1-/- mice harboring the 260.8 IgA producing hybridoma were colonized for 10 days with Bacteroides thetaiotaomicron VPI-5482.

通过对带有/不带有皮下接种260.8杂交瘤细胞的悉生（gnotobiotic）重组激活基因1敲除（Rag1-/-）小鼠进行对照实验，结果揭示：该免疫球蛋白A（IgA）既不会影响脆弱拟杆菌（B. thetaiotaomicron）的种群密度，也不会抑制260.8表位的产生，但可通过与宿主固有免疫应答减弱特征相符的方式调控细菌的基因表达。
实验整体设计如下：将C57BL/6野生型小鼠、C57BL/6J Rag1-/-小鼠，以及携带有可分泌260.8型IgA的杂交瘤细胞的C57BL/6J Rag1-/-小鼠，均用脆弱拟杆菌（Bacteroides thetaiotaomicron）VPI-5482菌株定殖10天。