microRNA levels data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells. microRNA levels data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells

To investigate the molecular mechanism of the hypopigmentation observed in Dicer KO mice, Dicer knockdown was realised in vitro in normal C57BL/6 mouse melanocyte Melan-a cells. Transient transfection of Melan-a cells with siRNA directed against Dicer reduced Dicer protein levels to approximately 40% of that of siScr Melan-a cells 24 hours after transfection. We analyzed the miRnome of Melan-a cells 24 and 48 hours after transfection with siDicer or siScr to understand the molecular mechanisms involved in Dicer-dependent melanocyte migration. Overall design: We extracted total RNA from mouse melanocytes Melan-a cells 24 and 48 hours after transfection of siRNA against Dicer (siDicer) or a siRNA control (siScr) and performed microarray-based miRnome analysis. Three biological replicates were used for each condition.

为探究Dicer基因敲除（Dicer KO）小鼠所呈现的色素减退的分子机制，我们在正常C57BL/6小鼠黑素细胞Melan-a中体外实施了Dicer敲低实验。采用靶向Dicer的小干扰RNA（small interfering RNA，siRNA）对Melan-a细胞进行瞬时转染，转染后24小时，细胞内Dicer蛋白水平可降至阴性对照siScr转染组的约40%。为明确依赖Dicer的黑素细胞迁移相关分子机制，我们分别在siDicer或siScr转染后24小时与48小时，对Melan-a细胞的微小RNA组（miRnome）开展检测分析。实验整体设计：我们从经靶向Dicer的siRNA（siDicer）或阴性对照siRNA（siScr）转染24小时、48小时的小鼠黑素细胞Melan-a中提取总RNA，并进行基于微阵列（microarray）的miRnome分析。每个实验条件均设置3次生物学重复（biological replicates）。