DNA methylation profiling in breast cancer discordant identical twins. Homo sapiens

We obtained a comprehensive DNA methylation profile of 15 breast cancer discordant twins, using the high resolution Infinium HumanMethylation450 BeadChip platform (450K, Illumina), previously established to reliably detect methylation changes of more than 450,000 CpG sites. To provide insight into the temporal and causal relationships and predictive potential, samples from breast cancer patients before (7) and after diagnosis (8) were also analyzed. Using whole blood from 15 twin pairs discordant for breast cancer and high-resolution (450k) DNA methylation analysis we identified 403 differentially methylated CpG sites including known and novel potential breast cancer genes. Overall design: 30 Samples

本研究采用高分辨率Infinium人类甲基化450K微珠芯片（Infinium HumanMethylation450 BeadChip，Illumina）平台，对15对乳腺癌不一致双生子开展全面的DNA甲基化图谱分析；该平台此前已被证实可稳定检测超过45万个CpG位点的甲基化变化。
为探究时间关联、因果关系及预测潜力，本研究同时分析了7例乳腺癌患者确诊前及8例确诊后的样本。
基于15对乳腺癌不一致双生子的全血样本，并结合高分辨率（450K）DNA甲基化分析技术，本研究共鉴定出403个差异甲基化CpG位点，其中涵盖已知及潜在的新型乳腺癌相关基因。
实验整体设计：共30份样本