Data from Nicolle et al. LC-HRMS study of Streptomyces sp. AgN23 Culture Media Extract. Study of AgN23 exometabolome and analysis of Arabidopsis metabolomic responses to the bacteria

This archive compiles several datasets related to studies of Streptomyces sp. AgN23 interaction with Arabidopsis thaliana. Ultra-high-performance liquid chromatography-high-resolution MS (UHPLC-HRMS) analyses were performed on a Q Exactive Plus quadrupole (Orbitrap) mass spectrometer, equipped with a heated electrospray probe (HESI II) coupled to a U-HPLC Ultimate 3000 RSLC system (Thermo Fisher Scientific, Hemel Hempstead, United Kigdom). For each biological sample, the RAW file obtained in ESI+ and ESI- mode were retrieved from the Xcalibur version 4.4 software and are deposited in separate sub-folders termed "RawPos" and "RawNeg". Each experimental cohort is grouped in a folder where the biological repeats can be retrieved, as well as QC (Quality Check, pool of all samples from the cohort), Blank samples and eventual alternative control such as Bennett, the mock control media of Streptomyces sp. AgN23. The details regarding samples preparation, analytic parameters and mass spectrometry, statistical treatment and visualization of the data will be made available in the publication relating to this archive. The folder " AgN23-WT_AgN23-pSC004" contains chromatograms related to metabolomic study of Wild-type and pSC004-1, pSC004-10, pSC004-16 and pSC004-22 mutants of Streptomyces sp. AgN23. The folder " Col-0_AgN23" contains chromatograms related to metabolomic study of Arabidopsis thaliana Col-0 responses to colonization by Streptomyces sp. AgN23-WT. The folder " Col-0_pad3-1_AgN23" contains chromatograms related to metabolomic study of Arabidopsis thaliana Col-0 and the Arabidopsis pad3-1 mutant responses to colonization by Streptomyces sp. AgN23-WT. The folder " Col-0_pSC004" contains chromatograms related to metabolomic study of Arabidopsis thaliana Col-0 responses to colonization by Streptomyces sp. AgN23-WT and the AgN23 mutants pSC004-10 and pSC004-22. It should be noted that in the publication associated with this archive, the pSC004-1, pSC004-10, pSC004-16 and pSC004-22 mutants are referred to as ΔgbnB-1, ΔgbnB-2, ΔgbnB-3 and ΔgbnB-4, respectively.本归档汇编了与链霉菌（Streptomyces sp.）AgN23与拟南芥（Arabidopsis thaliana）互作研究相关的多组数据集。超高效液相色谱-高分辨质谱（Ultra-high-performance liquid chromatography-high-resolution MS, UHPLC-HRMS）分析采用搭载加热电喷雾探针（heated electrospray probe, HESI II）的Q Exactive Plus四极杆（Orbitrap）质谱仪，该系统与赛默飞世尔科技（Thermo Fisher Scientific，英国赫默尔亨普斯特德）的U-HPLC Ultimate 3000 RSLC系统联用。针对每个生物学样本，其在正离子模式（ESI+）与负离子模式（ESI-）下获得的RAW文件均从Xcalibur 4.4版本软件中导出，并分别存储于名为"RawPos"与"RawNeg"的独立子文件夹中。每个实验队列均收纳于专属文件夹内，其中可获取生物学重复样本、QC（质量检查，Quality Check，即该队列所有样本的混合池）样本、空白样本，以及可选的对照样本，例如链霉菌AgN23的模拟接种培养基Bennett。有关样本制备、分析参数、质谱检测方法、统计处理流程与数据可视化方案的详细信息，将随本归档相关的学术论文一并公开。文件夹"AgN23-WT_AgN23-pSC004"包含链霉菌AgN23野生型（Wild-type, WT）及pSC004-1、pSC004-10、pSC004-16、pSC004-22突变体的代谢组学研究相关色谱图。文件夹"Col-0_AgN23"包含拟南芥Col-0生态型受链霉菌AgN23野生型定殖后的代谢组学研究相关色谱图。文件夹"Col-0_pad3-1_AgN23"包含拟南芥Col-0与pad3-1突变体受链霉菌AgN23野生型定殖后的代谢组学研究相关色谱图。文件夹"Col-0_pSC004"包含拟南芥Col-0生态型受链霉菌AgN23野生型及pSC004-10、pSC004-22突变体定殖后的代谢组学研究相关色谱图。需特别说明，在本归档关联的学术论文中，pSC004-1、pSC004-10、pSC004-16与pSC004-22突变体分别被标注为ΔgbnB-1、ΔgbnB-2、ΔgbnB-3与ΔgbnB-4。