Expression data from Human Umbilical Vein Endothelial Cell after influenza virus infection. Homo sapiens

This research focus primarily on the interaction between influenza virus and endothelial cell, then we used microarrays to observe global patterns of gene expression in Human Umbilical Vein Endothelial Cells after influenza virus infection and offer further insight into the interaction between endothelial cells and influenza viruses. We used microarrays to detail the global programme of gene expression and identified distinct classes of up-regulated genes during this process. Overall design: Human Umbilical Vein Endothelial Cells were selected at an early stage for RNA extraction and hybridization on Affymetrix microarrays. Then, we analysed the selected genes at expression level.

本研究主要聚焦于流感病毒与内皮细胞的相互作用，通过微阵列（microarrays）技术观测流感病毒感染后人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells）的全基因表达谱，为深入解析内皮细胞与流感病毒的相互作用提供进一步的研究见解。本研究利用微阵列技术详细阐明全基因表达程序，并在此过程中鉴定出多类显著上调的基因。
实验设计：选取流感病毒感染后早期阶段的人脐静脉内皮细胞进行RNA提取，并在Affymetrix微阵列上完成杂交实验，随后对筛选得到的基因开展表达水平分析。