Positive_Mortality_Effects_Predation_Data.xlsx

Four days prior to the beginning of the experiment, 500ml plastic containers were filled with 400ml recerse osmosis water, 1 g dried oak (<i>Quercus virginiana</i>) leaves, and 0.05g dried decorated crickets (Gryllodes sigillatus), and 100 µl microbial inoculum from rain-filled buckets. Containers were covered with plastic lids with holes punched for ventilation. Cohorts of 150 newly hatched Aedes aegypti were placed in each container and exposed to one of five predator treatments: no predator, Mesocyclops longisetus, Corethrella appendiculata, Anopheles barberi, and diverse. Single predator species treatments received 3 individual predators of the same species, and the diverse treatments received one of each species. All predators used were late instars. Predators were removed on day 6. 0.5g dried oak leaves and 0.025g dried decorated crickets were added on days 16 and 30 to replenish food resources for <i>A. aegypti. </i>Containers were checked daily for <i>A. aegypti </i>pupae which were placed in glass vials and the number of days from hatching to pupation recorded (Daystop in data). Pupae were checked daily for adults, which were removed and the sex and the number of days to adulthood (Daystoa in data file) recorded. Female adults were placed in a drying oven at 70C for &gt;48 hours, after which their wing lengths were measured.

实验开始前4日，将400ml反渗透水、1g干燥弗吉尼亚栎（Quercus virginiana）叶片、0.05g干燥装饰蟋（Gryllodes sigillatus）以及100μl取自积雨桶的微生物接种液注入500ml塑料容器中。容器加盖带有打孔通风孔的塑料盖。每个容器接入150只刚孵化的埃及伊蚊（Aedes aegypti），并施加5种捕食者处理之一：无捕食者、长肢中剑水蚤（Mesocyclops longisetus）、饰斑库蠓（Corethrella appendiculata）、巴伯按蚊（Anopheles barberi）以及多物种混合处理。单捕食者物种处理组投放3只同种晚期龄期捕食者，多物种混合处理组则投放上述3种捕食者各1只。所有受试捕食者均为晚期龄虫态。实验第6日移除所有捕食者。分别于第16日和第30日补充添加0.5g干燥弗吉尼亚栎叶片与0.025g干燥装饰蟋，以维持埃及伊蚊的食物资源。每日检查容器内的埃及伊蚊蛹，将蛹转移至玻璃小瓶中，并记录从孵化至化蛹的天数（数据中记为Daystop）。每日检查蛹是否羽化为成虫，记录成虫并记录其性别，同时记录从孵化至成虫羽化的天数（数据文件中记为Daystoa）。将雌性成虫置于70℃烘箱中干燥超过48小时，随后测量其翅长。