RESPAN Example Datasets and Results

Five example neuron datasets acquired with a Yokogawa W1 spinning disk confocal mounted on a Nikon Ti2 inverted microscope controlled with NIS-Elements. Images were acquired with a 100× 1.35 NA SR HP Plan Apo silicone immersion objective and a Prime BSI sCMOS camera (Teledyne Photometerics), resulting in pixel size of 65nm and a z-step size of 150nm.
Example expected results from RESPAN processing are also included.

本数据集包含5组神经元示例数据集，其成像系统为搭载于尼康Ti2倒置显微镜、由NIS-Elements软件控制的横河W1转盘式共聚焦显微镜。成像采用100倍、数值孔径1.35的SR HP Plan Apo硅浸物镜，搭配Teledyne Photometerics品牌的Prime BSI sCMOS相机，所得图像的像素尺寸为65nm，Z轴步进间距为150nm。此外，数据集中还附带了经RESPAN处理后的预期结果示例。