miRNA microarray of rTDSCs under mechanical treatment or not

Tendinopathy is a common disease in individuals whose tendons suffer from repetitive strains, such as athletes. The lack of knowledge regarding the pathological mechanisms causes inadequate prevention methods and low cure rate of this disease. Repetitive strain injury is thought to be the source of tendinopathy; therefore, we investigate the effect of mechanical loading on tendon and seek the ways to prevent and treat tendinopathy better. In our study, we found that the expression of a mechanosensitive miRNA, miR-337-3p, decreased in tendon-derived stem cells (TDSCs) under uniaxial cyclical mechanical loading with 10% elongation. In addition, miR-337-3p was negatively related to chondro-osteogenic differentiation of TDSCs, in which lower expression was found in human calcified tendons and rat tendinopathy samples.肌腱病（Tendinopathy）是一类好发于肌腱承受反复机械牵拉人群的常见疾病，运动员群体为高发人群。由于对其病理机制的认知不足，当前该病的预防手段匮乏，治愈率偏低。现有研究认为反复牵拉损伤是肌腱病的致病诱因，因此本研究旨在探究机械载荷对肌腱的调控作用，以期优化肌腱病的防治策略。本研究发现，在施加10%伸长率的单轴循环机械载荷条件下，肌腱源性干细胞（tendon-derived stem cells，缩写TDSCs）内机械敏感性微小RNA miR-337-3p的表达水平显著下调。此外，miR-337-3p与肌腱源性干细胞的软骨成骨分化呈负相关，且在人类钙化肌腱样本与大鼠肌腱病样本中，该微小RNA的表达水平均较低。