DNA repair in Mycoplasma gallisepticum

In the current study, we attempted to characterize the DNA repair system of Mycopalsma gallisepticum. We identified members of different DNA repair pathways based on genomic data and publications. We quantified mRNA of respective proteins per cell in a set of adverse conditions and identified most of them on the protein level. We showed induction of SOS-response in adverse conditions on a transcriptional level in the absence of a known regulator.

本研究旨在解析鸡毒支原体（Mycoplasma gallisepticum）的DNA修复系统。我们依托基因组数据与已发表文献，鉴定了该菌不同DNA修复通路的组成成员。我们在一系列逆境条件下，定量检测了各对应蛋白的单细胞mRNA表达水平，并在蛋白质层面验证了其中绝大多数蛋白的表达。我们证实，在缺失已知调控因子的前提下，逆境条件下的SOS响应（SOS-response）可在转录层面被诱导激活。