Methylation profiling classifies HIV-associated lymphoma

HIV-positive patients have a higher risk of non-Hodgkin's lymphoma with poor prognostic features. To characterize features of HIV-associated lymphoma, we compared the genome-wide DNA methylation profiles in between 11 HIV-associated and 20 non-HIV lymphoma samples by Illumina Infinium HumanMethylation450 BeadChip v1.0. Bisulfite-converted DNA from the 31 samples were analyzed to obtain the genome-wide methylation profile by using Illumina Infinium HumanMethylation450 BeadChip microarray.

HIV阳性患者罹患非霍奇金淋巴瘤（non-Hodgkin's lymphoma）的风险更高，且该类淋巴瘤多具有不良预后特征。为表征HIV相关淋巴瘤的特征，本研究采用Illumina Infinium人类甲基化450芯片v1.0（Illumina Infinium HumanMethylation450 BeadChip v1.0），对31例样本（含11例HIV相关淋巴瘤样本与20例非HIV相关淋巴瘤样本）的亚硫酸氢盐转化DNA（Bisulfite-converted DNA）进行检测，通过该芯片获取全基因组DNA甲基化谱并开展对比分析。