Differential counts of leukocytes in mouse BALF.

Aims
Asthma is characterized by chronic airway inflammation, persistent cough, wheezing, and dyspnea. This study aimed to evaluate the efficacy of Limosilactobacillus reuteri VHProbi® M07 (M07) administration in alleviate the asthma severity in a mice model.
Methods and results
In vitro studies confirmed that M07 can survive and proliferate within the gastrointestinal tract. BALB/c mice were administered M07 both before and after ovalbumin (OVA) challenge. Serum levels of OVA-specific immunoglobulin (Ig) E and IgG1, inflammatory cells and cytokines in bronchoalveolar lavage fluid were assessed, along with histopathological examination of lung tissue. Compared to the placebo (PLA) group, mice treated with M07 exhibited significantly lower levels of OVA-specific IgE and IgG1 (P < 0.01). The counts of eosinophils and neutrophils were also significantly reduced in both the pretreated (PRE) group and post-treated (POS) group compared with the PLA group (P < 0.01). Histological analysis of lung tissues verified the protective effects of M07 against inflammation, demonstrating reduced infiltration of inflammatory cells. Additionally, mice in the PRE and POS groups showed significantly increased levels of IL-10 (P < 0.01), and significantly decreased levels of IL-5, IL-13, MCP-1, eotaxin, and tumor necrosis factor-α (P < 0.01).
Conclusions
Oral administration of M07 mitigated key features of inflammatory responses in the OVA-induced mice asthma model. These findings suggest that M07 holds therapeutic potential for the treatment of allergic asthma.

研究目的：哮喘以慢性气道炎症、持续性咳嗽、喘息及呼吸困难为核心临床特征。本研究旨在评估罗伊氏乳杆菌（Limosilactobacillus reuteri）VHProbi® M07（以下简称M07）口服干预对小鼠哮喘模型病情严重程度的改善效果。
研究方法与结果：体外实验证实M07可在胃肠道内存活并增殖。本研究对BALB/c小鼠在卵清蛋白（OVA）致敏前后均给予M07干预。随后检测了血清中卵清蛋白（OVA）特异性免疫球蛋白E（IgE）与免疫球蛋白G1（IgG1）水平，支气管肺泡灌洗液中的炎症细胞计数与细胞因子水平，并对肺组织开展病理组织学检查。
与安慰剂（PLA）组相比，M07干预组小鼠的OVA特异性IgE及IgG1水平显著降低（P < 0.01）。相较于安慰剂组，预处理组（PRE）与后处理组（POS）小鼠的嗜酸性粒细胞、中性粒细胞计数均显著下降（P < 0.01）。肺组织病理学分析验证了M07的抗炎保护作用，可有效减少炎症细胞浸润。此外，预处理组与后处理组小鼠的白细胞介素10（IL-10）水平显著升高（P < 0.01），而白细胞介素5（IL-5）、白细胞介素13（IL-13）、单核细胞趋化蛋白1（MCP-1）、嗜酸性粒细胞趋化因子（eotaxin）及肿瘤坏死因子-α（TNF-α）水平均显著降低（P < 0.01）。
研究结论：口服M07可缓解卵清蛋白诱导的小鼠哮喘模型中炎症应答的关键病理特征。上述研究结果表明，M07在过敏性哮喘治疗领域具备潜在的临床应用价值。