Transcriptional response of tea roots of different genotypes to potassium. Transcriptional response of tea roots of different genotypes to potassium

Purpose: We investigated root foraging strategies for K of tea plants using a multi-layer split-root system by RNA-seq. Methods: One-year old tea cuttings were cultivated with the roots evenly planted on the two sides of the split root hydroponic box with a root canal. Three treatments were included to simulate the heterogeneous and homogeneous K environments. After 5d treatment, the roots on the two sides of the split root hydroponic box were collected separately and the RNA sequencing were analyzed by the Illumina Hiseq (2500, Illumina, San Diego, CA). Results: RNA-seq data had a linear relationship with qRT–PCR (r2=0.76), which confirmed the reliability of the RNA-seq data. Conclusions: Our study screened the key genes of tea root system to adapt to potassium heterogeneity. Overall design: Transcriptomic changes in low-potassium intolerant and tolerant tea plant (Camellia sinensis) genotypes under potassium heterogeneity.

研究目的：本研究采用多层分根系统结合RNA测序（RNA-seq），探究茶树针对钾元素（K）的根系觅养策略。
研究方法：选取一年生茶树扦插苗，将其根系均匀种植于带有根室的分根式水培箱两侧。设置3组处理，以模拟异质性与均质性钾离子环境。处理5天后，分别收集分根水培箱两侧的根系，采用Illumina Hiseq（2500，Illumina公司，美国加利福尼亚州圣地亚哥）进行RNA测序分析。
研究结果：RNA测序数据与实时定量PCR（qRT-PCR）结果呈线性相关（决定系数r²=0.76），证实了本次RNA测序数据的可靠性。
研究结论：本研究筛选出茶树根系适应钾离子异质性环境的关键基因。
整体实验设计：在钾离子异质性环境下，耐低钾与不耐低钾茶树（Camellia sinensis）基因型的转录组变化情况。