affy_syringae_malaga_athaliana-Role of different proteases in plant defence. Arabidopsis thaliana

affy_syringae_malaga_athaliana - affy_syringae_malaga_athaliana - - Pseudomonas syringae is a plant pathogenic bacterium that relies on a type III secretion system (T3SS) to cause disease in susceptible hosts and to trigger defence in resistant plants. The T3SS translocates effector proteins directly inside the plant cell. Some P. syringae pathovars translocate the effector HopZ1a, which is recognized in Arabidopsis, triggering the hypersensitive response (HR). This resistance does not depend on the usual defence pathways involved in resistance against other avirulence effectors, so it would be very helpful to know the transcriptomic events that take place in the context of a hypersensitive response triggered by HopZ1a. ULP genes codifies for plant SUMO-proteases. Recent studies by our group revealed that they could be involved in plant defense against microorganisms.-- We infiltrated Arabidopsis thaliana ecotype Columbia wild type with 10mM MgCl2 (as mock inoculation), the virulent pathogen Pseudomonas syringae pv. tomato (Pto), Pto expressing the effector HopZ1a and Pto expressing a HopZ1a derivative carrying a point mutation in a residue essential for the cystein-protease activity. We also grew ulp1c/ulp1d mutant plants to analyze them either without treatment or inoculated with MgCl2 (as mock) and Pto wt. Keywords: gene knock out,normal vs antisens mutant comparison Overall design: 24 arrays - ATH1

affy_syringae_malaga_athaliana - affy_syringae_malaga_athaliana - - 丁香假单胞菌（*Pseudomonas syringae*）是一类植物病原菌，其依赖III型分泌系统（T3SS）在感病宿主中引发病害，并在抗病植物中触发防御反应。III型分泌系统可将效应蛋白直接转运至植物细胞胞内。部分丁香假单胞菌致病型会分泌转运效应蛋白HopZ1a，该蛋白可在拟南芥（*Arabidopsis thaliana*）中被识别，进而诱发过敏反应（HR）。该抗性途径并不依赖于针对其他非毒性效应子的经典防御通路，因此解析HopZ1a触发的过敏反应过程中的转录组事件将具有重要研究意义。ULP基因编码植物SUMO蛋白酶，本课题组近期研究表明，这类蛋白酶可能参与植物对微生物的防御过程。
本实验采用浸润接种法，分别以10mM氯化镁（MgCl₂，空白接种对照）、致病性病原体丁香假单胞菌番茄致病变种（*Pseudomonas syringae* pv. *tomato*, Pto）、表达效应蛋白HopZ1a的Pto菌株，以及表达携带半胱氨酸蛋白酶活性必需残基点突变的HopZ1a突变体的Pto菌株，处理拟南芥哥伦比亚生态型野生型植株。此外，本研究还培育了ulp1c/ulp1d双突变体植株，分别设置未处理组、MgCl₂空白接种组以及野生型Pto接种组进行相关分析。
关键词：基因敲除（gene knock out）、野生型与反义突变体比较（normal vs antisens mutant comparison）
实验设计概况：共24个ATH1基因芯片