Stromal inflammation is a targetable driver of hematopoietic aging [Microarray analysis]. Stromal inflammation is a targetable driver of hematopoietic aging [Microarray analysis]

We performed integrated analyses of hematopoietic stem and progenitor cells as well as bone marrow stromal cells to better understand the underlying mechanisms of blood aging. To determine how the molecular state of multipotent progenitor (MPP) cells change with age we performed transcriptomic analyses of myeloid-biased MPP3 and lymphoid-biased MPP4 populations from young and old mice. Overall design: Microarray analysis of 3-5 biological replicated of the indicated MPP populations were isolated from young (8-10 weeks) and old (24 months) wild-type C57BL/6 mice. MPP3s are identified as Lin-/cKit+/Sca-1+/Flk2-/CD48+/CD150-, and MPP4s are identified as Lin-/cKit+/Sca-1+/Flk2+. Young controls listed here are also available under GSE68529.

为深入解析血液衰老的潜在分子机制，本研究对造血干细胞与造血祖细胞（hematopoietic stem and progenitor cells）以及骨髓基质细胞开展了整合分析。为明确多能祖细胞（multipotent progenitor, MPP）的分子状态随衰老发生的变化规律，本研究对年轻与老年小鼠的髓系偏倚MPP3群与淋系偏倚MPP4群开展了转录组分析。
整体实验设计：本研究从8~10周龄年轻野生型C57BL/6小鼠及24月龄老年野生型C57BL/6小鼠中分离目标MPP群，对每个群体设置3~5个生物学重复，开展微阵列（microarray）分析。MPP3的细胞表型鉴定为Lin⁻/cKit⁺/Sca-1⁺/Flk2⁻/CD48⁺/CD150⁻，MPP4则鉴定为Lin⁻/cKit⁺/Sca-1⁺/Flk2⁺。本研究中列出的年轻小鼠对照样本，亦可在GSE68529数据集下获取。