File S1 - PIAS1 Regulates Breast Tumorigenesis through Selective Epigenetic Gene Silencing

Contains the following files: Figure S1, Table S1 and Table S2. Figure S1. Validation of the polyclonal anti-PIAS1 antibody by immunofluorescence. MDA-MB231 cells containing control shRNA or PIAS1 shRNA2 were fixed by 3 different methods as indicated, followed by staining with polyclonal anti-PIAS1. FMA, formaldehyde. Table S1. Microarray analysis. Fold induction is defined as the ratio of the expression levels of a given gene in PIAS1 shRNA2 vs. control shRNA cells. Genes with greater than 10-fold induction under Stem Cell Media (SCM) condition are shown. Table S2. Primers used for Q-PCR, ChIP and methylation. (DOC)

本数据集包含以下文件：补充图S1（Figure S1）、补充表S1（Table S1）及补充表S2（Table S2）。补充图S1：通过免疫荧光（immunofluorescence）法验证多克隆抗PIAS1抗体（polyclonal anti-PIAS1 antibody）。将转染对照短发夹RNA（short hairpin RNA, shRNA）或PIAS1短发夹RNA2（PIAS1 shRNA2）的MDA-MB231细胞采用三种指定方法固定，随后以多克隆抗PIAS1抗体进行染色。其中FMA为甲醛（formaldehyde）的缩写。补充表S1：芯片微阵列分析（Microarray analysis）结果。诱导倍数（Fold induction）定义为目标基因在PIAS1 shRNA2转染细胞与对照shRNA转染细胞中的表达水平比值，本表格展示了在干细胞培养基（Stem Cell Media, SCM）培养条件下，诱导倍数大于10倍的基因。补充表S2：定量聚合酶链式反应（Quantitative Polymerase Chain Reaction, Q-PCR）、染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）及甲基化（methylation）实验所用引物序列。文件格式为DOC。