Do flower-colonizing microbes influence floral evolution? A test with fast-cycling Brassica

Pollinators are thought to be the main drivers of floral evolution. Flowers are also colonized by abundant communities of microbes that can affect the interaction between plants and their pollinators. Very little is known, however, about how flower-colonizing microbes influence floral evolution. Here we performed a six-generation experimental evolution study using fast-cycling Brassica rapa, in which we factorially manipulated the presence of pollinators and flower microbes to determine how pollinators and microbes interact in driving floral evolution. We measured the evolution of six morphological traits, as well as plant mating system and flower attractiveness. Only one of the six traits (flower number) evolved in response to pollinators, while microbes did not drive the evolution of any trait, nor did they interact with pollinators in driving evolution of morphological traits. Moreover, we did not find evidence that pollinators or microbes affected the evolution of flower attractiven..., , , # Do flower-colonizing microbes influence floral evolution? A test with fast-cycling Brassica

[https://doi.org/10.5061/dryad.cjsxksnf9](https://doi.org/10.5061/dryad.cjsxksnf9)

Data and code to analyse the effect of pollinators and microbes on the evolution of fast-cycling *Brassica rapa* in an experimental evolution study.

## Description of the data and file structure

There are three datasets:

\- \"Floral_traits\"

\- \"Autonomous-selfing\"

\- \"Choice_assays_brassica\"

**Floral_traits**

This dataset provides information on six plant traits measured on individuals of *Brassica rapa* from three different generations of experimental evolution (1, 7, and 8) and four treatments (presence or absence of pollination by bumblebees and inoculation by microbes, in a factorial design). This dataset was used with the code \"Floral_traits_analysis_SR\". Missing values in the dataset are identified as \"NA\".

Generation: Generation of experimental evolution for which the traits were measured.

Replic...

传粉者（pollinators）被认为是花部演化的主要驱动力。花部同时定殖有丰富的微生物群落，这些微生物能够影响植物与其传粉者之间的互作。然而，目前人们对定殖于花部的微生物如何影响花部演化仍知之甚少。本研究以快速世代白菜型油菜（Brassica rapa）为材料，开展了为期6个世代的实验演化研究，通过析因设计调控传粉者与花部微生物的存在与否，以解析二者在驱动花部演化过程中的互作模式。本研究测定了6个形态性状的演化情况，同时还测定了植物的交配系统与花部吸引力。结果显示，6个性状中仅花数量1个性状响应传粉者而发生演化；微生物并未驱动任何性状的演化，也未与传粉者互作以调控形态性状的演化。此外，本研究未发现传粉者或微生物影响花部吸引力演化的证据...
# 定殖于花部的微生物是否影响花部演化？基于快速世代白菜型油菜的验证实验
https://doi.org/10.5061/dryad.cjsxksnf9
本数据集与配套代码用于分析实验演化研究中，传粉者与花部微生物对快速世代白菜型油菜演化的影响。

## 数据集与文件结构说明
本数据集包含三个子数据集：
- "Floral_traits"（花部性状数据集）
- "Autonomous-selfing"（自主自交数据集）
- "Choice_assays_brassica"（芸苔属选择试验数据集）

**花部性状数据集**
本数据集包含从实验演化的3个世代（第1、7、8世代）的白菜型油菜个体中测得的6个植物性状信息，以及4种析因设计处理组（是否有熊蜂传粉、是否接种微生物）。本数据集配合代码"Floral_traits_analysis_SR"进行分析。数据集中的缺失值以"NA"标识。

世代：用于性状测定的实验演化世代。
Replic...