Chd4 deficiency effect on mouse embryonic stem cells. Mus musculus

Our study reveal that Chd4 is essential for stemness maintenance of ESCs. As Chd4 deficient ESCs show attentuated self-renewal ability and induced differentiation marker gene expression. To confirm the effect of Chd4 on the transcriptome profile of ESCs, we performed microarray analyses for Chd4 deficient ESCs. Overall design: We used the Tet-on inducible RNAi cell lines to achieve Chd4 knockdown. we performed microarray assays from 2 experimental groups with biological duplicate samples, Chd4i-1 cells treated with 5 ng/ml doxycyline for 3 days as the experimental group and without Dox treatment as the control group.

本研究揭示，Chd4对胚胎干细胞（Embryonic Stem Cells, ESCs）的干性维持至关重要。Chd4缺陷的胚胎干细胞表现出自我更新能力减弱，且分化标记基因的表达上调。为验证Chd4对胚胎干细胞转录组谱的影响，本研究对Chd4缺陷的胚胎干细胞开展了基因芯片（microarray）分析。总体实验设计：本研究采用Tet-on诱导型RNA干扰细胞系实现Chd4基因敲低。设置两个实验组，每组均设置生物学重复样本：以经5 ng/ml多西环素（Dox）处理3天的Chd4i-1细胞作为实验组，以未施加多西环素处理的Chd4i-1细胞作为对照组，并对两组样本进行基因芯片检测。