Profiling chromatin state by single-cell itChIP-seq [ESC_EpiLC]

We present a low-cost, generalizable ChIP-seq (itChIP), compatible to both low-input and single cells for profiling chromatin states. This method combines chromatin opening, simultaneous cellular indexing and chromatin tagmentation in a single tube. Single-cell itChIP data yield ~ 5000 unique reads per cell, sufficiently defining cell identifies and subpopulations of a given cell type. Our results demonstrate that itChIP is a generalizable technology for single-cell chromatin profiling of samples limited to ultra-low number of cells. Overall design: Based on Tn5 transposition of chromatin, the generalizable itChIP combines chromatin opening, simultaneous cellular indexing and chromatin tagmentation in a single tube.

我们报道了一种低成本、可通用的染色质免疫共沉淀测序（ChIP-seq）技术（itChIP），可适配低起始量样本与单细胞样本，用于染色质状态谱分析。该技术可在单管内同步完成染色质开放、细胞索引标记与染色质转座片段化操作。单细胞itChIP测序数据每个细胞可产生约5000条唯一读段，足以明确给定细胞类型的细胞身份与亚群组成。本研究结果证实，itChIP是一项可通用的技术，适用于超少量细胞样本的单细胞染色质状态谱分析。实验整体设计：基于染色质的Tn5转座反应，通用型itChIP技术在单管内整合了染色质开放、同步细胞索引标记与染色质转座片段化流程。