Effect of the gain-of-function mutation of CXCR4 on the molecular identity of murine Skeletal Stromal/Stem Cells (SSC) and OsteoProgenitor Cells (OPC)

In order to perform transcriptional analyses of two stromal subsets carrying the gain-of-function mutation of CXCR4, bulk RNA Sequencing (RNA-Seq) was conducted on SSC and OPC sorted from WT (Cxcr4+/+) , heterozygous (Cxcr4+/1013) and homozygous (Cxcr41013/1013) mutant mice for Cxcr4. Overall design: Comparative analysis of gene expression profiling using the RNA-seq data for Cxcr4+/+, Cxcr4+/1013 and Cxcr41013/1013 SSC or OPC

为对两种携带CXCR4功能获得性突变的基质细胞亚群开展转录组分析，研究人员对来自Cxcr4基因野生型（Cxcr4+/+）、杂合突变型（Cxcr4+/1013）及纯合突变型（Cxcr41013/1013）小鼠的分选所得SSC与少突胶质前体细胞（oligodendrocyte progenitor cells, OPC）进行了批量RNA测序（RNA-Seq）。实验整体设计：基于上述不同Cxcr4基因型小鼠的SSC或OPC的RNA-seq数据，开展基因表达谱的比较分析。