The HDAC Inhibitor Sodium Butyrate Prevents Allergic Rhinitis and Alters lncRNA and mRNA Expression Profiles in the Nasal Mucosa of Mice

Our previous research confirmed that histone deacetylase inhibitor sodium butyrate (NaB), delivered intranasally, has exhibits therapeutic effects on a mouse model of allergic rhinitis (AR). However, whether it is effective on AR when administered orally and prophylactically, as well as its potential effects on gene expression, remained unknown. Methods: In this study, we investigated the preventive effect of NaB on AR when added to the diet of newly weaned mice and evaluated changes in lncRNA and mRNA expression profiles in the nasal mucosa. Mice were randomly divided into three groups as follows: C group (control group, no treatment), AR group (treated with ovalbumin [OVA]), and NaB+AR group (treated with both OVA and NaB). The NaB+AR group was given NaB in their feed (30 g/kg), whereas the other two groups were fed normal feed from 3 weeks to 6 weeks of age. At 7 weeks of age, OVA administration was initiated to induce AR in the AR and NaB+AR groups. Then, behavioral assessments, western blotting, and gene analysis were performed. Mice were randomly divided into three groups(n = 30 per group) as follows: C group (control group, no treatment), AR group (treated with ovalbumin [OVA]), and NaB+AR group (treated with both OVA and NaB). The NaB+AR group was given NaB in their feed (30 g/kg), whereas the other two groups were fed normal feed from 3 weeks to 6 weeks of age. mice were sacrificed under anesthesia, and the nasal mucosa was preserved in liquid nitrogen for further analysis.

我们既往的研究证实，经鼻腔给药的组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitor）丁酸钠（sodium butyrate, NaB）对变应性鼻炎（allergic rhinitis, AR）小鼠模型具有治疗作用。然而，其经口预防性给药对变应性鼻炎是否有效，以及其对基因表达的潜在影响，目前仍不明确。
方法：本研究将丁酸钠添加至刚断奶小鼠的饲料中，探讨其对变应性鼻炎的预防作用，并检测鼻黏膜组织中长链非编码RNA（long non-coding RNA, lncRNA）与信使RNA（messenger RNA, mRNA）的表达谱变化。
将小鼠随机分为3组，具体如下：对照组（C组，不予任何处理）、变应性鼻炎模型组（AR组，以卵清蛋白[ovalbumin, OVA]造模）、丁酸钠干预+变应性鼻炎模型组（NaB+AR组，同时给予卵清蛋白与丁酸钠处理）。NaB+AR组小鼠的饲料中添加30 g/kg的丁酸钠，其余两组小鼠自3周龄至6周龄均饲喂普通饲料。
于7周龄时，对AR组与NaB+AR组小鼠启动卵清蛋白给药以诱导变应性鼻炎模型。随后开展行为学评估、蛋白质印迹（western blotting）检测与基因分析。
本研究再次将小鼠随机分为3组（每组30只），具体如下：对照组（C组，不予任何处理）、变应性鼻炎模型组（AR组，以卵清蛋白[ovalbumin, OVA]造模）、丁酸钠干预+变应性鼻炎模型组（NaB+AR组，同时给予卵清蛋白与丁酸钠处理）。NaB+AR组小鼠的饲料中添加30 g/kg的丁酸钠，其余两组小鼠自3周龄至6周龄均饲喂普通饲料。
对小鼠实施麻醉并处死，采集其鼻黏膜组织并置于液氮中保存，以待后续分析。