Data of the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain"

Data files for the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain." <br> <br> ******************************* <br> ****** GLOBAL ANALYSIS<br> ******************************* <br> <br>The compressed folder "./exemple_gel_1.zip" is an example of analysis of a gel done with CLIQS (http://totallab.com/home/cliqs/). <br> "img_7061_converted.tif" is the analysed picture. <br> "IMG_7061.1dxml" is the CLIQS database containing the position of the lanes, the Rf lines and the "grimaces". For details see (1). <br> "export_without_bg" and "export_with_bg" are containing the intensity profiles exported by CLIQS and used as input for the R-functions published in (1). <br> <br>The file "./global_analysis_nobg_H_Q.txt" is the output of the the R-functions published in (1). This is the dataset corresponding to the global analysis of this paper. <br> Column "nom":X[gel number]_[number of the lane in the gel, excluding the number of the lane] <br> Columns 1 to 34: intensity of the reference bands in the corresponding lane (row in the table). <br> Column "replicate": ID of the biological replicate of the experimental evolution. <br> Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. <br> Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) <br> <br> ********************************* <br> ****** SPECIFIC ANALYSIS<br> ********************************* <br> <br>./specific_analysis_LbGAP2.txt <br> Column "nom": X[gel number]_[number of the lane in the gel, excluding the number of the lane] <br> Column "replicate": ID of the biological replicate of the experimental evolution. <br> Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. <br> Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) <br> Column "LbGAP2_intens_western": Raw intensity of the western-blot against LbGAP2 <br> Column "intens_mediane_global_analysis": Median intensity of the reference bands of the globale analysis used to correct the raw intensity of the western-blot against LbGAP2 (see the material and method). <br> <br>./specific_analysis_LbGAP_LbSPN.txt <br> Column "nom": X[gel number]_[number of the lane in the gel, excluding the number of the lane] <br> Column "replicate": ID of the biological replicate of the experimental evolution. <br> Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. <br> Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) <br> Column "LbGAP": Presence or absence of the protein LbGAP (LbGAP is a dominant marker) <br> Column "LbSPNm": Presence or absence of the protein LbSPNm (LbSPN is a co-dominant marker) <br> Column "LbSPNy": Presence or absence of the protein LbSPNy (LbSPN is a co-dominant marker) <br> <br>(1) : Mathé Hubert, H., Gatti, J.-L., Colinet, D., Poirié, M., &amp; Malausa, T. (2015). Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom. Molecular Ecology Resources, 15, 1120–1132. http://doi.org/10.1111/1755-0998.12389 <br> <br><br>

本数据集为发表论文《Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain.》配套的数据文件。

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****** 全局分析
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压缩文件夹`./exemple_gel_1.zip`为使用CLIQS（http://totallab.com/home/cliqs/）完成的凝胶电泳图谱分析示例。
`img_7061_converted.tif`为经CLIQS分析的目标图像文件。
`IMG_7061.1dxml`为CLIQS数据库文件，存储了泳道位置、Rf线及CLIQS软件定义的“grimaces”参数，详细说明参见文献(1)。
`export_without_bg`与`export_with_bg`为CLIQS导出的蛋白条带强度分布文件，可作为文献(1)中所述R函数的输入数据。

文件`./global_analysis_nobg_H_Q.txt`为文献(1)中所述R函数的输出结果，为本论文全局分析对应的数据集。
各列说明如下：
- `nom`列：命名格式为`X[凝胶编号]_[凝胶内泳道编号]`（注：此处排除重复泳道的编号标识）
- 第1至34列：对应表格行（即泳道）内参考条带的强度值
- `replicate`列：实验进化的生物学重复样本标识ID
- `generation`列：实验进化的世代数，其中F1为ISm与ISy的杂交后代
- `hote`列：寄生蜂发育的宿主品系（YR=抗药性宿主；SY=敏感宿主；sen=维持ISm与ISy品系的宿主：Nasr）

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****** 特异性分析
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### 文件`./specific_analysis_LbGAP2.txt`
各列说明如下：
- `nom`列：命名格式为`X[凝胶编号]_[凝胶内泳道编号]`（注：此处排除重复泳道的编号标识）
- `replicate`列：实验进化的生物学重复样本标识ID
- `generation`列：实验进化的世代数，其中F1为ISm与ISy的杂交后代
- `hote`列：寄生蜂发育的宿主品系（YR=抗药性宿主；SY=敏感宿主；sen=维持ISm与ISy品系的宿主：Nasr）
- `LbGAP2_intens_western`列：针对LbGAP2的蛋白质免疫印迹（Western blot）原始强度值
- `intens_mediane_global_analysis`列：全局分析中参考条带的中位强度值，用于校正针对LbGAP2的蛋白质免疫印迹原始强度值（详见材料与方法部分）

### 文件`./specific_analysis_LbGAP_LbSPN.txt`
各列说明如下：
- `nom`列：命名格式为`X[凝胶编号]_[凝胶内泳道编号]`（注：此处排除重复泳道的编号标识）
- `replicate`列：实验进化的生物学重复样本标识ID
- `generation`列：实验进化的世代数，其中F1为ISm与ISy的杂交后代
- `hote`列：寄生蜂发育的宿主品系（YR=抗药性宿主；SY=敏感宿主；sen=维持ISm与ISy品系的宿主：Nasr）
- `LbGAP`列：蛋白LbGAP的存在与否（LbGAP为显性遗传标记）
- `LbSPNm`列：蛋白LbSPNm的存在与否（LbSPN为共显性遗传标记）
- `LbSPNy`列：蛋白LbSPNy的存在与否（LbSPN为共显性遗传标记）

文献(1)：
Mathé Hubert, H., Gatti, J.-L., Colinet, D., Poirié, M. 及 Malausa, T. (2015). 一维蛋白质图谱个体变异的统计分析及其在生态学中的应用：以寄生蜂毒液为例. *Molecular Ecology Resources*, 15, 1120–1132. http://doi.org/10.1111/1755-0998.12389