Arabidopsis LSM1 and DCP5 proteins aid evasion of translational repression during Cauliflower Mosaic Virus infection

Processing bodies are tightly connected with the RNA silencing machinery. Deletion of processing body components leads to succeptibility of Cauliflower Mosaic Virus to translational repression. Here, we investigated if this succeptibility is mediated through differential sRNA targeting of the viral genome. Overall design: 16 sRNA libraries from total RNA of Arabidopsis rosettes in duplicates of CaMV infected Col-0, lsm1, dcp5 and rdr6 plants with their respective double mutants. Controls include non-infected Col-0 and rdr6 plants.

处理小体（processing bodies）与RNA沉默机制（RNA silencing machinery）存在紧密关联。敲除处理小体组分会使花椰菜花叶病毒（Cauliflower Mosaic Virus，CaMV）对翻译抑制的敏感性升高。本研究旨在探究该敏感性是否通过病毒基因组的差异小RNA（small RNA，sRNA）靶向介导。总体实验设计：本研究共构建16个小RNA（small RNA，sRNA）文库，样品提取自拟南芥莲座叶的总RNA，涵盖感染花椰菜花叶病毒的Col-0、lsm1、dcp5、rdr6植株及其对应的双突变体，每组设置2个生物学重复；对照组为未感染花椰菜花叶病毒的Col-0与rdr6植株。