16S V3-V4 -based microbial profiling of duodenal mucosa samples obtained by esophagogastroduodenoscopy from patients affected by potential celiac disease and celiac disease. 16S V3-V4 -based microbial profiling of duodenal mucosa samples obtained by esophagogastroduodenoscopy from patients affected by potential celiac disease and celiac disease

Potential celiac diseasase (PCD) is characterized by a positive celiac disease (CD) serology and a normal small intestinal mucosa. This particular condition is usually considered a clinical challenge because, although its diagnostic criteria are clear, many questions are still unsettled. Therefore, given that PCD is a valuable biological model of the pathway leading to small intestinal mucosal damage in genetically predisposed individuals, the aim of our study is to evaluate whether immunological, microbial and lipid signatures could better characterize the PCD from the CD condition. Overall design: Total DNA was extracted from duodenal mucosa samples of 17 patients affected CD and 10 patients with PCD and amplicons of variable V3–V4 regions of the bacterial 16s rRNA gene were sequenced in paired end (2x300 cycle) on MiSeq Illumina platform.

潜在乳糜泻（Potential Celiac Disease, PCD）以乳糜泻（Celiac Disease, CD）血清学检测阳性且小肠黏膜形态正常为特征。该病症历来被视为临床诊疗难点，尽管其诊断标准已明确，但仍存在诸多尚未厘清的问题。鉴于PCD是探究遗传易感个体小肠黏膜损伤通路的宝贵生物学模型，本研究旨在评估免疫特征、微生物特征与脂质特征能否更精准地表征PCD与CD的差异。实验设计：本研究从17例确诊乳糜泻患者与10例潜在乳糜泻患者的十二指肠黏膜样本中提取总DNA，对细菌16S核糖体RNA（rRNA）基因的V3-V4可变区扩增子进行双端测序（2×300循环），测序平台为Illumina MiSeq平台。