Cell Data-Indenpendent acquisition LC-MS/MS

In this work, a microwell-chip was prepared and modified. The microwell-chip was used for extraction of metabolites and subsequent protein digestion. Next, direct electrospray ionization mass spectrometry (ESI-MS) was adopted for metabolome identification and a data independent acquisition (DIA)-MS approach was established for simultaneous proteome profiling and phosphoproteome analysis. In particular, application of this strategy provides a multi-omics view of cellular changes.

本研究制备并修饰了微孔芯片（microwell-chip）。该微孔芯片可用于代谢物提取及后续的蛋白质酶解。随后，采用直接电喷雾电离质谱（ESI-MS）完成代谢组鉴定，并建立了数据非依赖性采集质谱（DIA-MS）分析方法，可同步开展蛋白质组与磷酸化蛋白质组分析。尤为关键的是，本策略的应用可呈现细胞变化的多组学全貌。