Elevated Extra-cellular Matrix Signaling in Adipose Mesothelial Cells is Linked to Insulin Resistance in Obesity. Elevated Extra-cellular Matrix Signaling in Adipose Mesothelial Cells is Linked to Insulin Resistance in Obesity

Visceral adipose tissue fibrosis is linked to metabolic dysfunction in obesity; however, critical cell types that trigger and contribute to fibrosis in this depot are not completely understood. Whether adipose mesothelial cells (AMCs) play a role in pathologic obesity has been unclear. Overall design: Whole-genome expression profiling was conducted on AMCs isolated from a cohort of patients with varying degrees of metabolic disease undergoing bariatric surgery. Computational analysis of gene expression profiles was used to identify differentially expressed gene and gene signatures.

内脏脂肪组织纤维化（visceral adipose tissue fibrosis）与肥胖相关的代谢功能障碍存在紧密关联，但目前尚未完全明确该脂肪库中触发并促进纤维化的关键细胞类型。此前，脂肪间皮细胞（adipose mesothelial cells, AMCs）是否参与病理性肥胖的发生发展仍未可知。
总体实验设计：本研究对接受减重代谢手术、合并不同程度代谢疾病的患者队列中分离获取的脂肪间皮细胞进行了全基因组表达谱分析。通过对基因表达谱开展计算分析，本研究成功鉴定出差异表达基因及特征基因集。