Identification of genes differentially expressed during half-head regeneration in Schmidtea mediterranea

We used Nimblegen arrays with design 2007-11-06_Smed_ESTs_4_exp to measure changes in gene expression during a timecourse of regeneration of one side of the head in Schmidtea mediterranea flatworms. Following amputation of one side of the head, samples were collected from both the regeneration blastema and non-regenerating side of the head on days 2, 3, and 4 post-amputation. Gene expression in these samples was compared to that in non-regenerating control samples collected at the time of amputation. Four independent biological replicates for each sample type were collected from non-regenerating control animals and from the blastemas of regenerating animals on days 2, 3, and 4 post-amputation. Two independent biological replicates were collected from the non-regenerating side of the head on days 2, 3, and 4 post-amputation. Each probe is spotted in duplicate on the array, providing us with two simultaneously processed technical replicates for each biological sample.

本研究采用设计编号为2007-11-06_Smed_ESTs_4_exp的尼姆博金（Nimblegen）基因芯片，检测地中海真涡虫（Schmidtea mediterranea）单侧头部再生过程中的基因表达时序变化。在对涡虫单侧头部实施截肢处理后，本研究分别于截肢后第2、3、4天，从再生芽基（blastema）以及头部未再生侧采集样本。将上述样本的基因表达水平，与截肢即刻采集的未再生对照样本进行对比分析。针对未再生对照样本，以及截肢后第2、3、4天再生个体的芽基样本，每种样本类型均设置4次独立生物学重复。而截肢后第2、3、4天头部未再生侧的样本，则设置2次独立生物学重复。本阵列中每个探针均以重复点样形式排布，为每个生物学样本提供两份同步处理的技术重复。