Microarray expression analysis of Aquaporin-9 (AQP9) knockout effect in C57Bl/6 db/db (obese) mice

The experiment measures liver-tissue gene expression in Aquaporin-9 wildtype and knockout mice, in the leptin receptor deficient, obese C57Bl/6 mice. The knockout strategy has been published here PMID:17360690. Mice were 17-19 weeks old at tissue harvesting and provided a standardized control diet (Altromin #20000033; 22.0% protein and 5% fat) during the final 2 weeks of the experiment. Furthermore, the mice were removed from food for the final 4 hours before tissue harvesting.本实验以瘦素受体缺陷型肥胖C57BL/6小鼠为对象，检测其水通道蛋白-9（Aquaporin-9）野生型与基因敲除型小鼠的肝组织基因表达水平。该基因敲除策略已在PMID:17360690文献中发表。小鼠于组织取材时的周龄为17至19周，且在实验的最后两周喂食标准化对照饲料（Altromin #20000033；蛋白质含量22.0%，脂肪含量5%）。此外，小鼠在组织取材前4小时禁食。