Single-cell RNA sequencing reveals immune microenvironment changes induced by Fe-Shikonin treatment in SCC7 tumors

Single-cell RNA sequencing was performed on tumor tissues obtained from SCC7-bearing C3H mice to investigate the effects of Fe-Shikonin treatment on the tumor immune microenvironment. Tumor samples were collected from a phosphate-buffered saline (PBS)-treated control group and a Fe-Shikonin-treated group. In the deposited dataset, files designated as “CON1” correspond to tumor samples from the PBS control group, whereas files designated as “H1” correspond to tumor samples from the Fe-Shikonin treatment group. Single-cell libraries were constructed using the DNBSEQ-TaiM 4RS instrument (MGI) and DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) kit (Qingdao MGI Tech Co., Ltd).All libraries were sequenced on the DNBSEQ-T7RS platform (MGI) with paired-end reads.

本研究对携带SCC7肿瘤的C3H小鼠的肿瘤组织开展单细胞RNA测序（single-cell RNA sequencing），以探讨Fe-Shikonin治疗对肿瘤免疫微环境的影响。肿瘤样本分别取自磷酸盐缓冲液（phosphate-buffered saline, PBS）处理的对照组与Fe-Shikonin处理组。在本提交的数据集内，标记为"CON1"的文件对应磷酸盐缓冲液对照组的肿瘤样本，而标记为"H1"的文件则对应Fe-Shikonin治疗组的肿瘤样本。单细胞文库构建采用了DNBSEQ-TaiM 4RS仪器（MGI）以及DNBelab C系列高通量单细胞RNA文库制备试剂盒V3.0（TaiM 4）（青岛MGI科技有限公司）。所有文库均在DNBSEQ-T7RS测序平台（MGI）上以双端测序模式完成测序。