Supplementary information for GIL: A python package for designing custom indexing primers

Supplementary information for GIL: A python package for designing custom indexing primers. Final_Indexes.zip contains the output from GIL run with default parameters. The first plate of generated indexing primers were ordered and used to create 44 Illumina sequencing libraries. BCL files from a MiSeq Nano run of the 44 libraries were successfully demultiplexed with bcl2fastq using the sample sheets generated by GIL. Analysis of sequences that were not demultiplexed successfully to determine deletion frequency in index sequences can be found in demultiplexing_tests.zip, along with relevant fastq and stats files.GIL配套补充信息：一款用于设计定制化索引引物的Python软件包。Final_Indexes.zip 存储了以默认参数运行GIL所得到的输出结果。该工具生成的第一板索引引物已完成订购，并被用于构建44个Illumina测序文库。针对这44个文库开展MiSeq Nano测序所得的BCL文件，已通过GIL生成的样本表，借助bcl2fastq工具成功完成解多重处理。用于统计索引序列缺失频率的未成功解多重序列分析结果，以及相关FASTQ文件与统计文件，均可从demultiplexing_tests.zip中获取。