Proteomic analysis of mitoUFD binding partners

This project is to identify the binding partners of the OMM anchored ubiquitin-fusion degradation substrate (mitoUFD) in comparison with the mitoGFP control that lacks the ubiquitin fusion as the degradation signal. In addition, we performed RNAi knockdown for bcat-1, lars-1 and gcn-2 compared to the L4440 RNAi control to identify the changes of mitoUFD binding partners upon disruption of leucine catabolism or sensing.

本项目旨在鉴定锚定在线粒体外膜（Outer Mitochondrial Membrane, OMM）的泛素融合降解底物（mitoUFD）的结合伴侣，并以缺失泛素融合降解信号的mitoGFP对照组作为参照进行对比分析。此外，本研究以L4440 RNAi为空白对照，对bcat-1、lars-1与gcn-2实施RNA干扰敲低实验，以此探究亮氨酸代谢或感知受干扰时，mitoUFD结合伴侣的变化情况。