Hellbender skin transcriptome

In this study, we explored the efficacy of Bd vaccination on hellbender salamanders and functionally characterized host, host-associated microbiome, and pathogen throughout immunization and infection. We compared the function of hellbender skin, the skin microbiome, and Bd with a metatranscriptome approach at four experimental time points, (i) pre-vaccination and pre-infection, (ii) post-vaccination and pre-infection, (iii) early infection, and (iv) late infection.

本研究探究了Bd疫苗对隐鳃鲵（hellbender salamanders）的接种效力，并在免疫接种与感染的全过程中，对宿主、宿主相关微生物组及病原体开展功能表征。本研究采用宏转录组（metatranscriptome）技术，在四个实验时间点对隐鳃鲵皮肤功能、皮肤微生物组及Bd进行对比分析，四个实验时间点分别为：(i) 疫苗接种前与感染前；(ii) 疫苗接种后、感染前；(iii) 感染早期；(iv) 感染晚期。