miRNA expression profile in calcium oxalate (CaOx) nephrocalcinosis model.. miRNA expression profile in calcium oxalate (CaOx) nephrocalcinosis model.

Microarray analysis was used to assess the expression levels of miRNAs in six pairs of kidney tissues of calcium oxalate (CaOx) nephrocalcinosis mouse model and normal mouse kidney tissues.The animals used in the experiment were male 6-8 weeks old C57BL/6J mice. To establish a CaOx nephrocalcinosis mouse model, each mouse was intraperitoneally injected with saline or glyoxylate acid (Gly) (75 mg/kg/d, 200 μl) from day 1 to day 7. Animals were euthanized after 7 days, and kidney samples were collected at the time of euthanasia. Overall design: MiRNA expression profile in six pairs of kidney tissues of calcium oxalate (CaOx) nephrocalcinosis mouse and wild type mouse.

本研究采用微阵列（Microarray）分析技术，检测6对草酸钙（CaOx）肾钙质沉着症小鼠模型肾脏组织与正常小鼠肾脏组织中的微小RNA（miRNAs）表达水平。本实验所用动物为6-8周龄雄性C57BL/6J小鼠。为构建草酸钙（CaOx）肾钙质沉着症小鼠模型，实验小鼠于第1日至第7日每日腹腔注射生理盐水或乙醛酸（Gly），给药剂量为75 mg/kg/d，单次注射体积为200 μl。造模7日后对所有小鼠实施安乐死，并于安乐死即刻采集肾脏组织样本。实验整体设计：分析6对草酸钙（CaOx）肾钙质沉着症小鼠与野生型小鼠的肾脏组织微小RNA表达谱。