A hypomorphic Lsd1 allele results in heart development defects in mice

Microarray characterization of Lsd1 knock-outs compared to littermate controls Microarrays were employed to identify transcripts that were differentially modulated in the hearts of wild-type and Lsd1-hypomorphic late-stage embryos. Late-stage embryonic hearts were isolated from wild-type and Lsd1-hypomorphic hearts, in order to isolate RNA for hybridization on Affymetrix microarrays. We sought to identify gene products that are differentially regulated between the two genotypes, in order to explain the observed ventricular septal defects in the hypomorphic animals.与同窝对照相比的Lsd1基因敲除样本的微阵列（Microarray）表征分析。本研究采用微阵列技术，鉴定野生型与Lsd1低活性晚期胚胎心脏中差异调控的转录本。研究人员从野生型及Lsd1低活性晚期胚胎体内分离心脏组织，以提取RNA用于Affymetrix微阵列杂交实验。本研究旨在鉴定两种基因型间差异调控的基因产物，以此阐释低活性突变体动物中已观测到的室间隔缺损（ventricular septal defects）。