1_DrugVSCTL_Gene_differential_expression.xlsx

To glean insight into the genetic variation involved in acquired cisplatin resistance, we established an in vivo model of cisplatin-resistant cancer by administering a serial dose of cisplatin to mice bearing KB-3-1 human carcinoma cells. Through a whole transcriptome analysis using RNAseq, we set to identify significantly changed genes in tumors that acquired cisplatin resistance compared to treatment-naïve tumors.

为深入探究获得性顺铂耐药相关的遗传变异，我们向移植有KB-3-1人癌细胞的小鼠连续给予递增剂量的顺铂，构建了顺铂耐药癌症的体内模型。随后通过RNA测序（RNAseq）开展全转录组分析，旨在筛选出相较于初治（treatment-naïve）肿瘤，获得顺铂耐药的肿瘤中表达显著差异的基因。