Additional file 13 of Altered neuronal physiology, development, and function associated with a common chromosome 15 duplication involving CHRNA7

Additional file 13. Comparison of electrophysiological properties of neurons derived in vitro from the UC-M, AP, and UM iPSC models. (.xlsx). Data is shown for (A) all cells (cExNs and cINs), (B) cExNs, and (C) cINs. (A) Data columns for cells of each genotype show the median, first, and third quartiles, and count (# of cells) for each parameter for all cells recorded, by combining data from cExNs and cINs for each genotype. Columns to the right show neuronal cell type-specific data, by combining cExN data or cIN data for all three genotypes. Light red shading denotes Significant Difference by 2-WAY ANOVA on ranks with post hoc comparison by Student-Newman-Keuls method (p < 0.05) with genotype (UC-M : AP : UM) and neuronal cell type (cExN : cIN) as the two factors. (B-C) Values for (B) cExNs or (C) cINs of each genotype are shown. Tan shading denotes Significant Difference by 1-WAY ANOVA on ranks with post hoc comparison by Student-Newman-Keuls method (p < 0.05), with darker tan indicating that one of the genotypes was different from the other two and lighter tan indicating that two of the three genotypes were different from each other. Capacitance and input resistance were determined from 10 mV voltage steps from -80 mV. First spike threshold, amplitude and half-width were determined for the first action potential recorded at threshold. Maximum first frequency and maximum average firing frequency were determined for 800 msec depolarizing steps that elicited the maximum number of spikes under current clamp. Peak inward sodium current, steady-state outward potassium currents and currents evoked by ACh and choline were recorded under whole-cell voltage clamp. Values are provided.

附加文件13：源自UC-M、AP和UM诱导多能干细胞（induced pluripotent stem cell, iPSC）模型的体外培养神经元电生理特性比较（.xlsx格式）。数据分别展示了(A) 所有细胞（皮质兴奋性神经元cortical excitatory neurons, cExNs和皮质抑制性神经元cortical inhibitory neurons, cINs）、(B) 皮质兴奋性神经元以及(C) 皮质抑制性神经元的相关结果。(A) 针对各基因型的细胞，数据列展示了整合各基因型皮质兴奋性与抑制性神经元记录数据后，所有被测细胞各参数的中位数、第一四分位数、第三四分位数以及细胞计数（细胞总数）；右侧列则为神经元细胞类型特异性数据，整合三种基因型的皮质兴奋性神经元数据或皮质抑制性神经元数据得到。浅红色标注代表通过双向秩和方差分析（2-WAY ANOVA on ranks）结合Student-Newman-Keuls法进行事后多重比较所得的显著性差异（p < 0.05），分析的两个因素分别为基因型（UC-M : AP : UM）与神经元细胞类型（cExN : cIN）。(B-C) 分别展示了各基因型皮质兴奋性神经元(B)或皮质抑制性神经元(C)的数值。棕褐色标注代表通过单向秩和方差分析（1-WAY ANOVA on ranks）结合Student-Newman-Keuls法进行事后多重比较所得的显著性差异（p < 0.05）：深棕褐色表示某一种基因型与另外两种存在显著差异，浅棕褐色表示三种基因型中的两种彼此存在显著差异。电容与输入电阻通过-80 mV电位基础上施加10 mV电压阶跃的方式测得。首个动作电位阈值、幅度及半宽，通过阈值条件下记录到的首个动作电位计算得到。最大初始发放频率与最大平均发放频率，通过可诱发最大锋电位数的800 ms去极化阶跃电流钳实验测得。峰值内向钠电流、稳态外向钾电流以及乙酰胆碱（acetylcholine, ACh）与胆碱诱发的电流，通过全细胞膜片钳电压钳模式记录。所有相关数值均已提供。