Comparison of the DNA methylation of in vitro cultured bovine SAM-treated blastocysts and non-treated blastocysts

Epigenetics (DNA methylation) profiling of bovine in vitro cultured expanded blastocysts (EB) comparing control non-treated expanded blastocysts with SAM-treated expanded blastocysts. S-Adenosyl methionine (SAM) is the global methyl donor providing methyl Two-condition experiment, bovine non-treated expanded blastocysts (pools of 10) vs bovine SAM-treated expanded blastocysts. Four biological replicates of each tissue were hybridized to four two-color arrays in a dye-balanced design.

本数据集针对牛体外培养扩张囊胚（EB）开展表观遗传学（DNA甲基化）谱分析，对比未处理对照扩张囊胚与S-腺苷甲硫氨酸（SAM）处理的扩张囊胚。S-腺苷甲硫氨酸（SAM）是提供甲基的全局甲基供体。本双条件实验分别为牛未处理扩张囊胚（10枚为一组的混合样本）与牛SAM处理扩张囊胚。每组样本设置4个生物学重复，采用染料平衡设计将其与4张双色微阵列（two-color arrays）进行杂交。