SNHG5 promotes colorectal cancer cell survival by counteracting STAU1-mediated mRNA destabilization

We functionally characterize SNHG5 as an important lncRNA, which could constitute a new therapeutic target in colorectal cancer, and provide a novel mechanism of action for a cytoplasmic lncRNA Overall design: RNA pull down of SNHG5 in HCT-116 cells and subsequent sequencing of the transcriptional interactome

本研究对SNHG5进行功能表征，证实其为一类重要的长链非编码RNA（long non-coding RNA，lncRNA），可作为结直肠癌的新型治疗靶点，同时为细胞质定位的长链非编码RNA提供了全新的作用机制。实验整体设计：在HCT-116细胞中开展针对SNHG5的RNA下拉实验（RNA pull-down），随后对其转录互作组进行测序。