Detection of mature murine T and B-cells following serial transplantation of protein treated bone marrow cells in irradiated Rag-1−/− mice.

Cohorts of Rag-1−/− mice were irradiated with given a transplant of 103 bone marrow cells expanded with Tat-MYC and Tat-Bcl-2. Mice were analyzed 8 weeks post-transplant for mature murine T and B-cells. Bone marrow cells were collected from the Tat-MYC/Tat-Bcl-2 treated HSPC chimaeric mice, and 106 whole bone marrow cells were used for generating a second set of chimaeric mice. Likewise, this was also done a third time. FACS results were quantified and presented in this table across all of the mice in the initial transplant and two serial transplants that were carried out.Detection of mature murine T and B-cells following serial transplantation of protein treated bone marrow cells in irradiated Rag-1−/− mice.

将经Tat-MYC与Tat-Bcl-2扩增的1×10³个骨髓细胞移植给经辐照的Rag-1基因敲除小鼠（Rag-1−/− mice）以构建实验队列。于移植后8周，对各组小鼠的成熟T、B淋巴细胞进行检测分析。从经Tat-MYC/Tat-Bcl-2处理的造血干细胞（HSPC）嵌合小鼠体内收集骨髓细胞，取1×10⁶个全骨髓细胞用于构建第二批嵌合小鼠；同理重复该操作以构建第三批嵌合小鼠。本表格对初始移植及两次连续移植中所有受试小鼠的流式细胞术（FACS）检测结果进行定量统计与展示。经辐照的Rag-1基因敲除小鼠接受蛋白处理的骨髓细胞连续移植后成熟T、B淋巴细胞的检测分析。