Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type (WT) and E4BP4 Overexpressed Liver Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of liver-specific E4BP4 overexpression under mouse albumin promoter on the liver glucose and lipid metabolism. Methods: We generated transgenic mice (TG) with liver-specific E4BP4 overexpression. Livers were isolated at Zeitgeber Time (ZT) 2 or 14 from transgenic mice and WT littermates and total RNA was extracted. Liver RNA profiles were generated by deep sequencing for four groups with three mouse samples each. Results: Compared with the livers from the WT mice, those from Transgenic mice featured 290 differentially expressed genes (DEGs) (106 at ZT2, 134 at ZT14, and 14 at both times). Conclusions: Lipid metabolism might be altered in the transgenic liver. Liver mRNA profiles of 8-week old wild type mice and transgenic mice with liver-specific E4BP4 overexpression

研究目的：下一代测序（Next-generation Sequencing, NGS）技术革新了细胞通路的系统性分析。本研究旨在评估小鼠白蛋白启动子介导的肝脏特异性E4BP4过表达对肝脏糖脂代谢的影响。研究方法：我们构建了肝脏特异性过表达E4BP4的转基因小鼠（Transgenic Mice, TG）。分别于Zeitgeber时间（Zeitgeber Time, ZT）2及ZT14时点，从转基因小鼠及野生型（Wild Type, WT）同窝对照小鼠中分离肝脏组织并提取总RNA。对四组样本（每组包含3只小鼠的肝脏样本）进行深度测序，以获取肝脏转录组图谱。研究结果：与野生型小鼠的肝脏组织相比，转基因小鼠肝脏中共鉴定出290个差异表达基因（Differentially Expressed Genes, DEGs），其中ZT2时点有106个，ZT14时点有134个，两个时点共有的差异表达基因共14个。研究结论：转基因小鼠肝脏的脂质代谢可能发生改变。本数据集包含8周龄野生型小鼠及肝脏特异性过表达E4BP4的转基因小鼠的肝脏mRNA转录组数据。