Topical hydrogel containing Achyrocline satureioides oily extract (free and nanocapsule) has anti-inflammatory effects and thereby minimizes irritant contact dermatitis

Abstract Inflammatory dermatoses are prevalent worldwide, with impacts on the quality of life of patients and their families. The aim of this study was to determine the anti-inflammatory effects of Achyrocline satureioides oily extracts and nanocapsules on the skin using a mouse model of irritant contact dermatitis induced by croton oil, and a skin inflammation model induced by ultraviolet B (UVB) radiation. The mice were treated with 15 mg/ear oily extract (HG-OLAS) or nanocapsules (HG-NCAS) of A. satureioides incorporated into Carbopol® 940 hydrogels. We found that HG-OLAS and HG-NCAS formulations reduced ear edema in croton oil-induced lesions with maximum inhibitions of 54±7% and 74±3%, respectively. HG-OLAS and HG-NCAS formulations decreased ear edema induced by UVB radiation (0.5 J/cm2), with maximum inhibitions of 68±6% and 76±2% compared to the UVB radiation group, respectively. HG-OLAS and HG-NCAS modulated myeloperoxidase (MPO) activity after croton oil induction. Furthermore, croton oil and UVB radiation for 6 and 24 h, respectively, stimulated polymorphonuclear cells infiltration. The topical treatments reduced inflammatory processes, as shown by histological analysis. Together, the data suggest that topical application of A. satureioides oily extracts and nanocapsules produced antiedematogenic and anti-inflammatory effects. They constitute a compelling alternative for treatment of skin injuries.

摘要 炎症性皮肤病（inflammatory dermatoses）在全球范围内高发，对患者及其家属的生活质量造成显著负面影响。本研究旨在借助巴豆油（croton oil）诱导的刺激性接触性皮炎小鼠模型与紫外线B（ultraviolet B，UVB）辐射诱导的皮肤炎症模型，评估香脂艾克琳草（Achyrocline satureioides）油提取物与纳米胶囊的皮肤抗炎功效。实验中将浓度为15 mg/耳的香脂艾克琳草油提取物（HG-OLAS）或纳米胶囊（HG-NCAS）添加至卡波姆®940水凝胶中，对小鼠进行局部给药处理。结果显示，HG-OLAS与HG-NCAS制剂可减轻巴豆油诱导皮损处的耳肿胀，最大抑制率分别为54±7%与74±3%；二者亦可降低UVB辐射（0.5 J/cm²）诱导的耳肿胀，相较于UVB辐射组，最大抑制率分别为68±6%与76±2%。HG-OLAS与HG-NCAS可调节巴豆油诱导后的髓过氧化物酶（myeloperoxidase，MPO）活性。此外，巴豆油与UVB辐射分别处理6小时与24小时后，可促进多形核细胞浸润；组织学分析结果证实，局部给药可有效抑制炎症进程。综合上述实验数据，香脂艾克琳草油提取物与纳米胶囊经局部给药后可发挥抗肿胀与抗炎作用，有望成为皮肤损伤治疗的极具潜力的替代方案。