Inosine enhances mitochondria respiration to promote cancer survival under starvation. Inosine enhances mitochondria respiration to promote cancer survival under starvation

To investigate mechanism of inosine promotes the survival and metabolism of MDA-MB-231 cells under starvation conditions, MDA-MB-231 cells were treated with inosine and glucose for 12h under starvation conditions. We then performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB-231 cells under three different treatments（-G-Q,Inosine,Glucose）. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MDA-MB-231 cells under three different treatments（-G-Q,Inosine,Glucose）.

为探究肌苷（inosine）在饥饿条件下促进MDA-MB-231细胞存活与代谢的分子机制，本研究将MDA-MB-231细胞置于饥饿环境中，分别施加肌苷处理、葡萄糖处理以及无额外添加的饥饿对照处理，处理时长均为12小时。随后，针对三种不同处理组（-G-Q组、肌苷组、葡萄糖组）的MDA-MB-231细胞进行RNA测序（RNA-seq），并基于所得数据开展基因表达谱分析。实验整体设计：对上述三种处理组的MDA-MB-231细胞的RNA-seq数据开展对比基因表达谱分析。