Transcriptomic dataset for Sardina pilchardus: assembly, annotation and tissue expression

European sardine or pilchard is a planktonic small pelagic fish present from the North Sea in Europe to the coast of Senegal in the North of Africa, and across the Mediterranean sea to the Black Sea. Due to its high contents in proteins and lipids, the fishing of this species is of great nutritional and economical value. Although their importance in the food basket, little is known about the molecular mechanisms present in this species involved in the protein and lipid synthesis. We collected nine tissues of Sardina pilchardus and reconstructed the transcriptome of this species. We obtained 198,597 transcripts, from which 68,031 are protein-coding. Quality assessment was performed by back-mapping reads to the transcriptome and by searching for Single Copy Orthologs. Additionally, Gene Ontology, KEGG and eggNOG annotations were retrieved for most of the protein-coding genes. Finally, each library was quantified in terms of Transcripts per Million to discover their expression patterns.<br>

欧洲沙丁鱼（European sardine or pilchard）为浮游性小型中上层鱼类，分布范围从欧洲北海延伸至非洲北部塞内加尔沿岸，并横跨地中海至黑海。
因其蛋白质与脂质含量较高，该物种的捕捞产业具备极高的营养与经济价值。
尽管该物种在食品保障体系中具有重要地位，但学界对其参与蛋白质与脂质合成的分子机制仍知之甚少。
本研究采集了欧洲沙丁鱼（Sardina pilchardus）的9种组织，并重构了该物种的转录组（transcriptome）。
最终获得198597条转录本，其中68031条为蛋白编码转录本。
通过将测序reads回贴至转录组以及搜索单拷贝同源基因（Single Copy Orthologs），完成了转录组的质量评估。
此外，本研究为绝大多数蛋白编码基因获取了基因本体（Gene Ontology）、京都基因与基因组百科全书（KEGG）以及eggNOG注释信息。
最后，以每百万转录本数（Transcripts per Million, TPM）对各测序文库进行定量分析，以解析其基因表达模式。