Additional file 8: of Plant pectin acetylesterase structure and function: new insights from bioinformatic analysis

Sequence-structure comparison between the amino acid sequence of AtPAE8 and a human palmitoleoyl-protein carboxylesterase (4UYU_A) threaded with FUGUE [75]. The catalytic triad in 4UYU is shown in dark red; other important amino acid residues involved in 4UYU activity are depicted in light red; amino acids involved in the substrate binding pocket are shown in blue. A cluster of 12 or 13 cysteine residues is in green [30]. The amino acid sequence of each protein does not contain the signal peptide. (XLSX 69 kb)

采用FUGUE穿线法[75]对AtPAE8的氨基酸序列与人类棕榈油酰基蛋白质羧酸酯酶（4UYU_A）开展序列-结构比对。其中，4UYU的催化三联体以深红色标注；参与4UYU酶活性的其他重要氨基酸残基以浅红色标注；参与底物结合口袋的氨基酸以蓝色标注。由12或13个半胱氨酸残基组成的簇以绿色标注[30]。两种蛋白质的氨基酸序列均不含信号肽。（XLSX 69 kb）