Figure10a-d.nd2

Figure 10. S. aureus MRSA biofilms grown without P. aeruginosa cell-free conditioned media (CFCM) (A – D) or with CFCM (E – H) for 24 hours to assess the viability of bacterial cells re-maining. Wheat Germ Agglutinin Alexa Fluor 647 was used to identify S. aureus cells (A and E). Live (B and F) and Dead (C and G) were visualized using SYTO 9 and Propidium Iodide, respec-tively, and captured simultaneously. Merged images were constructed by overlaying all channels to visualize the extent to which S. aureus cells were killed by CFCM (D and H). Viability was determined using ImageJ Biofilm viability checker[29].

图10. 金黄色葡萄球菌（S. aureus）耐甲氧西林菌株（MRSA）生物膜在无铜绿假单胞菌（P. aeruginosa）无细胞条件培养基（CFCM）（A-D）或添加CFCM（E-H）的条件下培养24小时，以评估残留细菌细胞的活力。使用小麦胚芽凝集素Alexa Fluor 647标记金黄色葡萄球菌细胞（A和E）。活菌（B和F）与死菌（C和G）分别通过SYTO 9和碘化丙啶（Propidium Iodide）可视化，并同步捕获图像。通过叠加所有通道构建合并图像，以观察CFCM对金黄色葡萄球菌细胞的杀伤程度（D和H）。细菌活力通过ImageJ生物膜活力检查器[29]测定。