Evaluating the effect of CovR phosphorylation on group A streptococcus global gene expression. Evaluating the effect of CovR phosphorylation on group A streptococcus global gene expression

We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus Overall design: There were 8 strains analyzed, each in quadruplicate replicates: 1) wild-type GAS serotype M1; 2) covS-E281A GAS serotype M1; 3) covS-T284A GAS serotype M1 4) covR-D53A GAS serotypep M1; 5) wild-type GAS serotype M3; 6) covS-E281A GAS serotype M3; 7.) covS-T284A GAS serotype M1; 8.) covR-D53A GAS serotypep M3

本研究旨在探究可改变CovR磷酸化水平的CovRS突变对A群链球菌（Group A Streptococcus, GAS）基因表达谱的影响。
实验设计概述：本次分析共纳入8株菌株，每株菌株均设置4次生物学重复：1）野生型A群链球菌血清型M1；2）covS-E281A突变型A群链球菌血清型M1；3）covS-T284A突变型A群链球菌血清型M1；4）covR-D53A突变型A群链球菌血清型M1；5）野生型A群链球菌血清型M3；6）covS-E281A突变型A群链球菌血清型M3；7）covS-T284A突变型A群链球菌血清型M1；8）covR-D53A突变型A群链球菌血清型M3。