ChIP-on-chip data from human ES cells-derived CD34+CD43+CD45low cells (hemogenic endothelium-like cells) overexpressing 3xFLAG-Sox17-ERT

Overexpression of transcription factor Sox17 in human ES cells-derived endothelial cells enhances expansion of hemogenic endothelium-like cells. Human ES cells were differentiated for 6 days in EBs, then CD34+CD43-CD45- endothelial cells were isolated, plated onto OP9 cells, and transduced with the 4OH-tamoxifen (4OHT)-inducible 3×FLAG-tagged Sox17-ERT retrovirus. The cells were seeded on OP9 stromal cells and cultured in the presence of 4OH-tamoxifen. At day 27 of the co-culture with OP9 cells, CD34+CD43+CD45low hemogenic endothelium-like cells overexpressing Sox17-ERT were collected by CD34 magnetic-activated cell sorting (MACS) and subjected to a ChIP-chip analysis.

在人类ES细胞来源的内皮细胞中过表达转录因子Sox17，可促进造血内皮样细胞的扩增。具体实验过程为：将人类ES细胞在胚状体（EBs）中诱导分化6天，随后分离得到CD34+CD43-CD45-内皮细胞，接种于OP9细胞层，并使用经4-羟基他莫昔芬（4OH-tamoxifen，4OHT）诱导的3×FLAG标签化Sox17-ERT逆转录病毒进行转导。将转导后的细胞接种于OP9基质细胞层，并在添加4-羟基他莫昔芬的培养基中培养。在与OP9细胞共培养第27天时，通过CD34磁激活细胞分选（MACS）收集过表达Sox17-ERT的CD34+CD43+CD45low造血内皮样细胞，并对其进行染色质免疫共沉淀芯片（ChIP-chip）分析。