First evidence of Whole Genome Sequencing of Mycobacterium tuberculosis complex for molecular distribution and drug susceptibility test made in Senegal. Mycobacterium tuberculosis

A prospective study was carried out in Senegal on patients TB positive. An extraction from positive cultures isolates was carried out then WGS using Illumina Miseq technology to characterize the species and lineage as well as identify the mutations that can induce the resistance of treatment confirming the significant presence of M. tuberculosis complex and lineages.

本研究在塞内加尔针对结核病阳性患者开展了一项前瞻性研究。研究人员对培养阳性的分离株进行提取后，采用因美纳（Illumina）MiSeq测序技术完成全基因组测序（Whole Genome Sequencing, WGS），以对菌种与谱系进行分型鉴定，并识别可引发治疗耐药性的突变，最终证实了结核分枝杆菌复合群（M. tuberculosis complex）及其谱系的显著存在。