The BAF complex does not mediate GLI repression (RNA-Seq)

Purpose: This study seeks to determine whether Smarcc1 co-regulates Hedgehog (HH) target genes in the limb. Methods: To determine if Smarcc1 is required to regulate HH targets, we used genomic approaches to identify Smarcc1-regulated genes within the forelimb. We performed bulk RNA-seq on individually genotyped forelimb-bud pairs at E11.5 (39-40s) on control (2 replicates) and PrxCre/+;Smarcc1c/c (2 replicates) embryos. From this experiment we identified 928 differentially expressed genes (FDR<0.05). Results: We find that while certain HH target genes are co-regulated by SMARCC1, the majority of HH target genes do not require Smarcc1. Bulk RNA-seq on individual E11.5 (39-40s) forelimb bud pairs from control (2 replicates) and PrxCre/+;Smarcc1c/c (2 replicates) embryos.研究目的：本研究旨在明确Smarcc1是否在肢体组织中共同调控刺猬信号（Hedgehog，HH）靶基因。 研究方法：为验证Smarcc1是否为调控HH靶基因所必需，本研究采用基因组学方法鉴定前肢中受Smarcc1调控的基因。我们对E11.5（39-40体节期）的单个基因分型前肢芽对样本进行批量RNA测序（bulk RNA-seq），其中对照组为2个生物学重复的胚胎，实验组为2个生物学重复的PrxCre/+;Smarcc1c/c基因型胚胎。经该实验共鉴定出928个差异表达基因（错误发现率<0.05，FDR<0.05）。 研究结果：本研究发现，尽管部分HH靶基因受SMARCC1共同调控，但大多数HH靶基因并不依赖Smarcc1。我们对E11.5（39-40体节期）的单个基因分型前肢芽对样本进行批量RNA测序，样本来自对照组（2个生物学重复）与PrxCre/+;Smarcc1c/c（2个生物学重复）胚胎。