RNAseq of CD31-/CD45- pneumocytes after 4 weeks of KRasG12V activation by tamoxifen

We report the RNAseq data obtained from 50.000-100.000 CD31-/CD45- pneumocytes isolated by FACS from mice harboring a normal dose or one extra copy of the Sirt1 gene, and a tamoxifen-inducible oncogenic KI alelle of KRasG12V after 4 weeks of tamoxifen treatment. Pneumocytes with the activated form of the inducible KRasG12V oncogene sere selected making use of the reporter gene LacZ (located next to the oncogene in the same polycistronic mRNA), by loading CD31-/CD45- pneumocytes with the LacZ-activated fuorogenic molecule FDG prior to FACS sorting. Overall design: Four replicates of each genetic group (Sirt1-WT and Sirt1-Tg) pneumocytes were used for this study. Sirt1-WT were used as reference controls.本研究报道了从携带正常剂量或额外1拷贝Sirt1基因、且经他莫昔芬处理4周后带有他莫昔芬诱导型致癌KRasG12V敲入（KI）等位基因的小鼠中，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分离得到的50000~100000个CD31阴性/CD45阴性肺上皮细胞的RNA测序（RNA-seq）数据。本研究通过将CD31-/CD45-肺上皮细胞与经LacZ激活的荧光底物FDG共孵育，在FACS分选前筛选出携带诱导型KRasG12V致癌基因激活形式的细胞；其中报告基因LacZ与该致癌基因位于同一多顺反子mRNA中且紧邻其旁。实验整体设计如下：本研究使用每个遗传组（Sirt1野生型（Sirt1-WT）与Sirt1转基因型（Sirt1-Tg））的肺上皮细胞各4个生物学重复，其中Sirt1-WT作为对照参考样本。